• Catalog Number:

  E1A0817A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of tyrosine 326 (N-D-YP-G-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Phospho-Tyr326) antibody detects endogenous levels of Akt only when phosphorylated at tyrosine 326.

• References:

  Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991). Matsubara A., Diabetologia 44:910-913(2001). Olsen J.V., Cell 127:635-648(2006). ----------------------------------------------------------------------------

• All Sites:

  Human: Tyr326; Mouse: Tyr326; Rat: Tyr326

Western blot analysis of extracts from mouse liver cells, using Akt (Phospho-Tyr326) antibody.