• Catalog Number:

  E1A0812A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RBL1 around the phosphorylation site of threonine 369 (P-S-TP-P-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P28749

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  107 kDa retinoblastoma-associated protein, PRB1, RBL1, Retinoblastoma-like protein 1, p107

• Specificity Sensitivity:

  RBL1 (Phospho-Thr369) antibody detects endogenous levels of RBL1 only when phosphorylated at threonine 369.

• References:

  Zhu L., Genes Dev. 7:1111-1125(1993). Deloukas P., Nature 414:865-871(2001). The MGC Project Team, Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Thr369; Mouse: Thr369

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using RBL1 (Phospho-Thr369) antibody. RBL1 (Phospho-Thr369) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.