• Catalog Number:

  E1A0811A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of threonine 821 (L-P-TP-P-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein

• Specificity Sensitivity:

  Rb (Phospho-Thr821) antibody detects endogenous levels of Rb only when phosphorylated at threonine 821.

• References:

  Hogg A., Oncogene 7:1445-1451(1992). Toguchida J., Genomics 17:535-543(1993). Dunham A., Nature 428:522-528(2004).

• All Sites:

  Human: Thr821; Mouse: Thr814; Rat: Thr792

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human testis tissue using Rb (Phospho-Thr821) antibody. Immunofluorescence analysis of HeLa cells, using Rb (Phospho-Thr821) antibody.