• Catalog Number:

  E1A0810A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 811 (L-K-SP-P-Y).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06400

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  P105-RB, PP105, PP110, RB-1, RB1, Retinoblastoma-associated protein

• Specificity Sensitivity:

  Rb (Phospho-Ser811) antibody detects endogenous levels of Rb only when phosphorylated at serine 811.

• References:

  Basta P., Biochim. Biophys. Acta 1132:154-160(1992). Hillier L.W., Nature 434:724-731(2005). Cenni V., Biochem. J. 363:537-545(2002).

• All Sites:

  Human: Ser811; Mouse: Ser804; Rat: Ser782

P-peptide - + Western blot analysis of extracts from K562 cells, using Rb (Phospho-Ser811) antibody. Immunofluorescence analysis of HuvEc cells, using Rb (Phospho-Ser811) antibody.