• Catalog Number:

  E1A0808A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKD2 around the phosphorylation site of serine 876 (R-I-SP-V-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q9BZL6

• Applications:

  WB: 1:500~1:1000 ELISA: 1:60000

• Other Names:

  EC 2.7.11.13, HSPC187, KPCD2, KPCO, PRKD2, Protein kinase C, D2 type, Protein kinase D2, Protein kinase D2 homolog, kinase PKD2, nPKC-D2

• Specificity Sensitivity:

  PKD2 (Phospho-Ser876) antibody detects endogenous levels of PKD2 only when phosphorylated at serine 876.

• References:

  Nicholas Obermüller, Am J Physiol Renal Physiol, Dec 1999; 277: 914. Rong Ma, Mol. Cell. Biol., Sep 2005; 25: 8285 - 8298. Dana R. Rundle, J. Biol. Chem., Jul 2004; 279: 29728 - 29739. Sabine Sturany, J. Biol. Chem., Aug 2002; 277: 29431 - 29436

• All Sites:

  Human: Ser876; Mouse: Ser873

Western blot analysis of extracts from NIH/3T3cells, treated with PMA (250ng/ml, 15mins), using PKD2 (Phospho-Ser876) antibody.