• Catalog Number:

  E1A0806A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKD1/PKC μ around the phosphorylation site of serine 205 (R-L-SP-N-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q15139

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  EC 2.7.11.13, KPCD1, PKC-mu, PKCM, PKD, PRKCM, PRKD1, Protein kinase C, mu type, Protein kinase D, kinase PKD1, nPKC-mu

• Specificity Sensitivity:

  PKD1/PKC μ (Phospho-Ser205) antibody detects endogenous levels of PKD1/PKC μ only when phosphorylated at serine 205.

• References:

  Lynn Pritchard, Hum. Mol. Genet., Nov 2000; 9: 2617 - 2627. Klaus B. Piontek, J. Am. Soc. Nephrol., Dec 2004; 15: 3035 - 3043. Saori Nishio, J. Clin. Invest., Apr 2005; 115: 910 - 918. Guanqing Wu, Hum. Mol. Genet., Aug 2002; 11: 1845 - 1854.

• All Sites:

  Human: Ser205; Mouse: Ser203;

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PKD1/PKC μ (Phospho-Ser205) antibody. Western blot analysis of extracts from NIH/3T3 cells treated with Anisomycin (25μg/ml, 30mins) (lane 1) and NIH/3T3 cells treated with H2O2 (100μM, 30mins) (lane 2), using PKD1/PKC μ (Phospho-Ser205) antibody.