• Catalog Number:

  E1A0805A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC ζ around the phosphorylation site of threonine 560 (Q-L-TP-P-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05513

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.11.13, KPCZ, PKC-zeta, PKC2, PRKCZ, Protein kinase C zeta type, kinase PKC-zeta, nPKC-zeta

• Specificity Sensitivity:

  PKC ζ (Phospho-Thr560) antibody detects endogenous levels of PKC ζ only when phosphorylated at threonine 560.

• References:

  Richard Magid, Circ. Res., Sep 2005; 97: 443 - 449. M Fujihara, J. Immunol., Feb 1994; 152: 1898. A. Banan, Am J Physiol Gastrointest Liver Physiol, May 2002; 282: 794. A. Banan, Am J Physiol Gastrointest Liver Physiol, Oct 2002; 283: 909.

• All Sites:

  Human: Thr560; Mouse: Thr560; Rat: Thr560

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PKC ζ (Phospho-Thr560) antibody. Western blot analysis of extracts from COS7cells, treated with PMA (125ng/ml, 30mins), using PKC ζ (Phospho-Thr560) antibody.