• Catalog Number:

  E1A0804A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC θ around the phosphorylation site of serine 695 (N-F-SP-F-M).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q04759

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.13; kinase PKC-theta; KPCT; nPKC-theta; PKC-theta; PKCQ; PRKCQ; PRKCT; Protein kinase C, theta type

• Specificity Sensitivity:

  PKC θ (Phospho-Ser695) antibody detects endogenous levels of PKC θ only when phosphorylated at serine 695.

• References:

  Chang J.D., J. Biol. Chem. 268:14208-14214(1993). Chang J.D., J. Biol. Chem. 269:31322-31322(1994). Baier G., J. Biol. Chem. 268:4997-5004(1993).

• All Sites:

  Human: Ser695; Mouse: Ser695

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC θ (Phospho-Ser695) antibody. Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using PKC θ (Phospho-Ser695) antibody.