• Catalog Number:

  E1A0803A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC-pan around the phosphorylation site of threonine 497 (T-S-TP-F-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17252/P05771/Q05655/Q02156/ P05129/P24723/Q04759/Q05513

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha; PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta; PKC-epsilon, PKCEA, PRKCE, epsilon type, kinase PKC-epsilon, nPKC-epsilon

• Specificity Sensitivity:

  PKC-pan (Phospho-Thr497) antibody detects endogenous levels of PKC-pan only when phosphorylated at threonine 497.

• References:

  Gevaert K., Nat. Biotechnol. 21:566-569(2003). Wissing J., Mol. Cell. Proteomics 6:537-547(2007). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Hillier L.W., Nature 434:724-731(2005).

• All Sites:

  Human: Thr497; Mouse: Thr497; Rat: Thr497

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC-pan (Phospho-Thr497) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKC-pan (Phospho-Thr497) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) antibody.