• Catalog Number:

  E1A0802A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC ε around the phosphorylation site of serine 729 (G-F-SP-Y-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02156

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  EC 2.7.11.13, KPCE, PKC-epsilon, PKCEA, PRKCE, Protein kinase C, epsilon type, kinase PKC-epsilon, nPKC-epsilon

• Specificity Sensitivity:

  PKC ε (Phospho-Ser729) antibody detects endogenous levels of PKC ε only when phosphorylated at serine 729.

• References:

  Basta P., Biochim. Biophys. Acta 1132:154-160(1992). Hillier L.W., Nature 434:724-731(2005). Cenni V., Biochem. J. 363:537-545(2002).

• All Sites:

  Human: Ser729; Mouse: Ser729; Rat: Ser729

Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC ε (Phospho-Ser729) antibody.