• Catalog Number:

  E1A0801A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC δ around the phosphorylation site of tyrosine 52 (T-M-YP-P-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05655

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  EC 2.7.11.13, KPCD, PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta

• Specificity Sensitivity:

  PKC δ (Phospho-Tyr52) antibody detects endogenous levels of PKC δ only when phosphorylated at tyrosine 52.

• References:

  Aris J.P., Biochim. Biophys. Acta 1174:171-181(1993). Ota T., Nat. Genet. 36:40-45(2004). Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Tyr52; Mouse: Tyr52; Rat: Tyr52

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PKC δ (Phospho-Tyr52) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using PKC δ (Phospho-Tyr52) antibody.

P-peptide - + Immunofluorescence analysis of NIH/3T3 cells, using PKC δ (Phospho-Tyr52) antibody.