• Catalog Number:

  E1A0800A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC α around the phosphorylation site of tyrosine 657 (F-S-YP-V-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17252

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha

• Specificity Sensitivity:

  PKC α (Phospho-Tyr657) antibody detects endogenous levels of PKC α only when phosphorylated at tyrosine 657.

• References:

  Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990). McSwine-Kennick R.L., J. Biol. Chem. 266:15135-15143(1991). Gevaert K., Nat. Biotechnol. 21:566-569(2003).

• All Sites:

  Human: Tyr657; Mouse: Tyr657; Rat: Tyr657

Western blot analysis of extracts from COLO205 cells, using PKC α (Phospho-Tyr657) antibody.