• Catalog Number:

  E1A0799A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of threonine 179and tyrosine 181 (E-M-TP-G-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16539

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  CRK1, CSAID binding protein, CSBP, CSBP1, CSBP2, Cytokine suppressive anti- inflammatory drug binding protein, EC 2.7.11.24, MAP kinase MXI2, MAP kinase p38, MAPK14, MAX-interacting protein 2, MK14, MXI2, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38, kinase p38-alpha

• Specificity Sensitivity:

  p38 MAPK (Phospho-Thr179+Tyr181) antibody detects endogenous levels of p38 MAPK only when phosphorylated at threonine 179 and tyrosine 181.

• References:

  Lee J.C., Nature 372:739-746(1994). Han J., Biochim. Biophys. Acta 1265:224-227(1995). Zervos A.S., Proc. Natl. Acad. Sci. U.S.A. 92:10531-10534(1995). --------

• All Sites:

  Human: Thr179+Tyr181; Mouse: Thr179+Tyr181; Rat: Thr179+Tyr181

Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using p38 MAPK (Phospho-Thr179+Tyr181) antibody.