• Catalog Number:

  E1A0793A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421 (E-F-SP-D-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13547

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  HD1, Histone deacetylase 1, RPD3L1

• Specificity Sensitivity:

  HDAC1 (Phospho-Ser421) antibody detects endogenous levels of HDAC1 only when phosphorylated at serine 421.

• References:

  Taunton J., Science 272:408-411(1996). Furukawa Y., Cytogenet. Cell Genet. 73:130-133(1996). Sparrow D.B., EMBO J. 18:5085-5098(1999).

• All Sites:

  Human: Ser421; Mouse: Ser421

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using HDAC1 (Phospho-Ser421) antibody.