• Catalog Number:

  E1A0792A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.3 around the phosphorylation site of serine 31 (A-P-SP-T-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84243

• Applications:

  IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  CG5825, H3.3Q, H3.A, H3.B, H33, H3F3A, H3F3B, HIS3.3A, HIS3.3B ORCG8989, HISH3-3Q, Histone H3.3

• Specificity Sensitivity:

  Histone H3.3 (Phospho-Ser31) antibody detects endogenous levels of Histone H3.3 only when phosphorylated at serine 31.

• References:

  Albig W., Genomics 30:264-272(1995). Wan D., Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004). Gregory S.G., Nature 441:315-321(2006).

• All Sites:

  Human: Ser31; Mouse: Ser31; Rat: Ser31

P-peptide - + Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) antibody.