• Catalog Number:

  E1A0788A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (V-D-YP-V-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13480

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  Growth factor receptor bound protein 2-associated protein 1

• Specificity Sensitivity:

  GAB1 (Phospho-Tyr659) antibody detects endogenous levels of GAB1 only when phosphorylated at tyrosine 659.

• References:

  Lisa S. Lock, Mol. Biol. Cell, Jun 2002; 13: 2132. Anders Kallin, J. Biol. Chem., Apr 2004; 279: 17897 - 17904. Mélanie Laramée, J. Biol. Chem., Mar 2007; 282: 7758 - 7769. Mariko Takahashi-Tezuka, Mol. Cell. Biol., Jul 1998; 18: 4109 - 4117.

• All Sites:

  Human: Tyr659; Mouse: Tyr660; Rat: Tyr659

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using GAB1 (Phospho-Tyr659) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using GAB1 (Phospho-Tyr659) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using GAB1 (Phospho-Tyr659) antibody.