• Catalog Number:

  E1A0784A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 383 (M-R-TP-L-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  CHEK2, CHK2, Cds1, Chk2, EC 2.7.11.1, RAD53, kinase Chk2

• Specificity Sensitivity:

  Chk2 (Phospho-Thr383) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383.

• References:

  Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741. Jinwoo Ahn, J. Biol. Chem., Dec 2002; 277: 48418 - 48426. Nabil H. Chehab, Genes & Dev., Feb 2000; 14: 278.

• All Sites:

  Human: Thr383; Mouse: Thr387; Rat: Thr386

P-peptide - + Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Phospho-Thr383) antibody. Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) antibody.