• Catalog Number:

  E1A0783A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Breast Tumor Kinase around the phosphorylation site of tyrosine 447 (T-S-YP-E-N).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13882

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

• Other Names:

  Breast tumor kinase, EC 2.7.10.2, PTK6, Tyrosine-protein kinase 6, Tyrosine-protein kinase brk

• Specificity Sensitivity:

  Breast Tumor Kinase (Phospho-Tyr447) antibody detects endogenous levels of Breast Tumor Kinase only when phosphorylated at tyrosine 447.

• References:

  Mitchell P.J., Oncogene 9:2383-2390(1994). Lee H.-Y., Mol. Cells 8:401-407(1998). Deloukas P., Nature 414:865-871(2001).

• All Sites:

  Human: Tyr447; Mouse: Tyr447

P-peptide - + Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Breast Tumor Kinase (Phospho-Tyr447) antibody. Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) antibody.