• Catalog Number:

  E1A0781A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human B-Raf around the phosphorylation site of serine 602 (V-K-SP-R-W).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15056

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  B-RAF, B-RAF proto-oncogene serine/threonine-protein kinase, B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, EC 2.7.1.37, EC 2.7.11.1, RAFB1, RMIL, RMIL serine/threonine-protein kinase, c-RMIL, kinase B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

• Specificity Sensitivity:

  B-Raf (Phospho-Ser602) antibody detects endogenous levels of B-Raf only when phosphorylated at serine 602.

• References:

  Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418. Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949. R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953. Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

• All Sites:

  Human: Ser602; Mouse: Ser639; Rat: Ser639

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human placenta tissue using B-Raf (Phospho-Ser602) antibody. Western blot analysis of extracts from NIH-3T3 cells, treated with EGF (200ng/ml, 30mins), using B-Raf (Phospho-Ser602) antibody.