• Catalog Number:

  E1A0780A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human B-Raf around the phosphorylation site of threonine 598 (L-A-TP-V-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15056

• Applications:

  IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  B-RAF, B-RAF proto-oncogene serine/threonine-protein kinase, B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, EC 2.7.1.37, EC 2.7.11.1, RAFB1, RMIL, RMIL serine/threonine-protein kinase, c-RMIL, kinase B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

• Specificity Sensitivity:

  B-Raf (Phospho-Thr598) antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

• References:

  Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418. Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949. R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953. Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

• All Sites:

  Human: Thr598; Mouse: Thr626; Rat: Thr636

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody.