• Catalog Number:

  E1A0778A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bloom Syndrome Protein around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P54132

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  Bloom's syndrome protein, DNA helicase, RecQ-like, type 2, EC 3.6.1.-, RECQ2, RECQL3, RecQ protein-like 3

• Specificity Sensitivity:

  Bloom Syndrome Protein (Phospho-Thr99) antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

• References:

  Ellis N.A., Cell 83:655-666(1995). Karow J.K., J. Biol. Chem. 272:30611-30614(1997). Pichierri P., EMBO J. 23:3154-3163(2004).

• All Sites:

  Human: Thr99

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using Bloom Syndrome Protein (Phospho-Thr99) antibody. Western blot analysis of extracts from HepG2 cells, using Bloom Syndrome Protein (Phospho-Thr99) antibody.