• Catalog Number:

  E1A0775A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BCL-XL around the phosphorylation site of threonine 47 (M-E-TP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q07817

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  Apoptosis regulator Bcl-X, BCL2L, BCL2L1, BCLX, BLC2L, Bcl-2-like 1 protein

• Specificity Sensitivity:

  BCL-XL (Phospho-Thr47) antibody detects endogenous levels of BCL-XL only when phosphorylated at threonine 47.

• References:

  KE Foreman, Am. J. Pathol., Sep 1996; 149: 795. Mariola Kurowska, J. Immunol., Aug 2002; 169: 1760. Yun Dai, Mol. Pharmacol., Dec 2003; 64: 1402. Charles Perkins, Blood, Feb 2000; 95: 1014 - 1022.

• All Sites:

  Human: Thr47; Mouse: Thr47; Rat: Thr47

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using BCL-XL (Phospho-Thr47) antibody. Western blot analysis of extracts from 293 cells, treated with UV (30mins), using BCL-XL (Phospho-Thr47) antibody.

P-peptide - + Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) antibody.