• Catalog Number:

  E1A0774A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 69 (A-R-TP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  Apoptosis regulator Bcl-2, BCL2

• Specificity Sensitivity:

  BCL-2 (Phospho-Thr69) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 69.

• References:

  H. Kurosawa, Am J Physiol Gastrointest Liver Physiol, Jun 1997; 272: 1587. JR Park, Blood, Aug 1995; 86: 868 - 876. A Plettenberg, Am. J. Pathol., Mar 1995; 146: 651. JM Ben-Ezra, Am. J. Pathol., Nov 1994; 145: 1036.

• All Sites:

  Human: Thr69

P-peptide - +

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using BCL-2 (Phospho-Thr69) antibody. Immunofluorescence analysis of HeLa cells, using BCL-2 (Phospho-Thr69) antibody.