• Catalog Number:

  E1A0773A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bax around the phosphorylation site of threonine 167 (F-G-TP-P-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q07812

• Applications:

  IF: 1:100~1:500 ELISA: 1:40000

• Other Names:

  Apoptosis regulator BAX, membrane isoform alpha, BAXA, Bcl2-associated X protein

• Specificity Sensitivity:

  Bax (Phospho-Thr167) antibody detects endogenous levels of Bax only when phosphorylated at threonine 167.

• References:

  Francesca Andriani. J Natl Cancer Inst, Sep 2001; 93: 1314 - 1324. H Martinez-Valdez. J. Exp. Med., Mar 1996; 183: 971. Joslyn K. Brunelle. J. Biol. Chem., Feb 2004; 279: 4305 - 4312.

• All Sites:

  Human: Thr167; Mouse: Thr167; Rat: Thr167

P-peptide - +

Immunofluorescence analysis of A549 cells, using Bax (Phospho-Thr167) antibody. Bax (Phospho-Thr167) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.