• Catalog Number:

  E1A0771A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ataxin 1 around the phosphorylation site of serine 776 (R-W-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P54253

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  ATX1, ATXN1, SCA1, Spinocerebellar ataxia type 1 protein, Spinocerebellar ataxia type 1 protein homolog

• Specificity Sensitivity:

  Ataxin 1 (Phospho-Ser776) antibody detects endogenous levels of Ataxin 1 only when phosphorylated at serine 776.

• References:

  Banfi S., Nat. Genet. 7:513-519(1994). Mungall A.J., Nature 425:805-811(2003). Quan F., Hum. Mol. Genet. 4:2411-2413(1995).

• All Sites:

  Human: Ser776; Mouse: Ser752

P-peptide - + Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Ataxin 1 (Phospho-Ser776) antibody. Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) antibody.