• Catalog Number:

  E1A0770A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P10398

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

• Other Names:

  A-RAF, A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF, ARAF1, EC 2.7.11.1, PKS, PKS2, Proto-oncogene Pks, Raf, kinase A-Raf

• Specificity Sensitivity:

  A-RAF (Phospho-Tyr301/302) antibody detects endogenous levels of A-RAF only when phosphorylated at tyrosine 301/tyrosine 302.

• References:

  Oliver G. Rössler. Am J Physiol Cell Physiol, May 2004; 286: C1118 - C1129. Marie A. Bogoyevitch. J. Biol. Chem., Nov 1995; 270: 26303. Weicheng Liu. J. Biol. Chem., Mar 2004; 279: 10167 - 10175. M Huleihel. Mol. Cell. Biol., Jul 1986; 6: 2655 - 2662.

• All Sites:

  Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using A-RAF (Phospho-Tyr301/302) antibody. Western blot analysis of extracts from HeLa cells, treated with PMA (125ng/ml, 30mins), using A-RAF (Phospho-Tyr301/302) antibody.

P-peptide - + Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr301/302) antibody.