• Catalog Number:

  E1A0769A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Arachidonate 5 lipoxygenase around the phosphorylation site of serine 271 (Q-L-SP-L-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P09917

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  5-lipoxygenase, ALOX5, Arachidonate 5-lipoxygenase, EC 1.13.11.34, LOG5, LOX5

• Specificity Sensitivity:

  Arachidonate 5 Lipoxygenase (Phospho-Ser271) antibody detects endogenous levels of Arachidonate 5 lipoxygenase only when phosphorylated at serine 271.

• References:

  RJ Boado, PNAS, Oct 1992; 89: 9044. K Ochi, J. Biol. Chem., May 1983; 258: 5754 - 5758. N Ueda, J. Biol. Chem., Jun 1986; 261: 7982 - 7988. Jagadananda Ghosh, PNAS, Oct 1998; 95: 13182.

• All Sites:

  Human: Ser272; Mouse: Ser272; Rat: Ser271

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Arachidonate 5 Lipoxygenase (Phospho-Ser271) antibody. Western blot analysis of extracts from HuvEc cells, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) antibody.