• Catalog Number:

  E1A0766A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPK β1 around the phosphorylation site of serine 181 (S-S-SP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q9Y478

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb ; PRKAB1; AMPK

• Specificity Sensitivity:

  AMPK β1 (Phospho-Ser181) antibody detects endogenous levels of AMPK β1 only when phosphorylated at serine 181.

• References:

  Carling D., Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases. Stapleton D., FEBS Lett. 409:452-456(1997). Yamagata K., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser182; Mouse: Ser182; Rat: Ser182

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK β1 (Phospho-Ser181) antibody. Western blot analysis of extracts from COS7 cells treated with PMA (125ng/ml, 30mins), using AMPK β1 (Phospho-Ser181) antibody.