Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human AhR around the phosphorylation site of serine 36 (N-P-SP-K-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
AhR (Phospho-Ser36) antibody detects endogenous levels of AhR only when phosphorylated at serine 36.
References:
Itoh S., Nucleic Acids Res. 21:3578-3578(1993).
Dolwick K.M., Mol. Pharmacol. 44:911-917(1993).
Ema M., J. Biochem. 116:845-851(1994).
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All Sites:
Human: Ser36; Mouse: Ser35; Rat: Ser35
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using AhR (Phospho-Ser36) antibody.
Western blot analysis of extracts from HepG2 cells, using AhR (Phospho-Ser36) antibody.