• Catalog Number:

  E1A0763A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ADAM 17 around the phosphorylation site of threonine 735 (P-Q-TP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P78536

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  ADA17, ADAM 17, CD156b antigen, CSVP, EC 3.4.24.86, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme, a disintegrin and metalloproteinase domain 17, snake venom-like protease

• Specificity Sensitivity:

  ADAM 17 (Phospho-Thr735) antibody detects endogenous levels of ADAM 17 only when phosphorylated at threonine 735.

• References:

  Moss M.L., Nature 385:733-736(1997). Patel I.R., J. Immunol. 160:4570-4579(1998). Diaz-Rodriguez E., Mol. Biol. Cell 13:2031-2044(2002). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Thr735; Mouse: Thr735; Rat: Thr735

Western blot analysis of extracts from K562 cells, treated with UV (5mins), using ADAM 17 (Phospho-Thr735) antibody.