• Catalog Number:

  E1A0762A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Actin-pan (α/γ) around the phosphorylation site of tyrosine 53/55 (D-S-YP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P60709/P63261

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  ACTA, ACTA1, ACTS, Actin, alpha skeletal muscle, Alpha-actin 1; ACTG, ACTG1, actin, cytoplasmic 2, gamma-actin

• Specificity Sensitivity:

  Actin-pan (α/γ) (Phospho-Tyr53/55) antibody detects endogenous levels of Actin-pan (α/γ) only when phosphorylated at tyrosine 53/55.

• References:

  A. J. MacQueen, Mol. Biol. Cell, Jul 2005; 16: 3247 - 3259. Isaac Rabinovitz, J. Cell Biol., Dec 1997; 139: 1873. Deanne H. Hryciw, Am J Physiol Renal Physiol, Jun 2005; 288: F1227 - F1235. Derek T. Warren, J. Cell Sci., Apr 2002; 115: 1703.

• All Sites:

  Human: Tyr55/Tyr53/Tyr53; Mouse: Tyr55/Tyr53/Tyr57

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin-pan (α/γ) (Phospho-Tyr53/55) antibody.