• Catalog Number:

  E1A0761A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PFKFB2 around the phosphorylation site of serine 483 (N-Y-SP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O60825

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, F262, PFK-2/FBPase-2, PFK-2/FBPase-2, heart type, fructose-2,6-bisphosphatase

• Specificity Sensitivity:

  PFKFB2 (Phospho-Ser483) antibody detects endogenous levels of PFKFB2 only when phosphorylated at serine 483.

• References:

  SJ Pilkis, J. Biol. Chem., May 1983; 258: 6135 - 6141. Tsuyoshi Furumoto, Plant Cell Physiol., Oct 2001; 42: 1044 - 1048. AD Colosia, J. Biol. Chem., Dec 1988; 263: 18669 - 18677. MR el-Maghrabi, J. Biol. Chem., Aug 1987; 262: 11714 - 11720.

• All Sites:

  Human: Ser483; Mouse: Ser486; Rat: Ser486

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PFKFB2 (Phospho-Ser483) antibody. Western blot analysis of extracts from 293 cells, treated with Heat shock, using PFKFB2 (Phospho-Ser483) antibody.