• Catalog Number:

  E1A0760A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 53BP1 around the phosphorylation site of serine 6 (T-G-SP-Q-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q12888

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1

• Specificity Sensitivity:

  53BP1 (Phospho-Ser6) antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 6.

• References:

  Iwabuchi K., J. Biol. Chem. 273:26061-26068(1998). The MGC Project Team; Genome Res. 14:2121-2127(2004). Iwabuchi K., Proc. Natl. Acad. Sci. U.S.A. 91:6098-6102(1994).

• All Sites:

  Human: Ser6; Mouse: Ser6; Rat: Ser11

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using 53BP1 (Phospho-Ser6) antibody. Western blot analysis of extracts from COS-7 cells, treated with insulin (0.01U/ml, 15mins), using 53BP1 (Phospho-Ser6) antibody.