• Catalog Number:

  E1A0759A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ/δ around the phosphorylation site of threonine 232 (S-D-TP-Q-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P63104

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ

• Specificity Sensitivity:

  14-3-3 ζ/δ (Phospho-Thr232) antibody detects endogenous levels of 14-3-3 ζ/δ only when phosphorylated at threonine 232.

• References:

  Alastair Aitken, J. Biol. Chem., Mar 1995; 270: 5706. L Kockel, Genes & Dev., May 1997; 11: 1140 - 1147. Martijn J. van Hemert, J. Cell Sci., Mar 2004; 117: 1411 - 1420. Anne-Sophie Vercoutter-Edouart, Cancer Res., Jan 2001; 61: 76 - 80.

• All Sites:

  Human: Thr232; Mouse: Thr232

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using 14-3-3 ζ/δ (Phospho-Thr232) antibody. Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using 14-3-3 ζ/δ (Phospho-Thr232) antibody.