• Catalog Number:

  E1A0758A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 θ/τ around the phosphorylation site of serine 232 (S-D-SP-A-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P27348

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Other Names:

  14-3-3 protein T-cell, 14-3-3 protein tau, 1433T, 143T, HS1 protein, YWHAQ

• Specificity Sensitivity:

  14-3-3 θ/τ (Phospho-Ser232) antibody detects endogenous levels of 14-3-3 θ/τ only when phosphorylated at serine 232.

• References:

  Nielsen P.J., im. Biophys. Acta 1088:425-428(1991). Leffers H., J. Mol. Biol. 231:982-998(1993). Gevaert K., Nat. Biotechnol. 21:566-569(2003).

• All Sites:

  Human: Ser232; Mouse: Ser232

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 14-3-3 θ/τ (Phospho-Ser232) antibody. Western blot analysis of extracts from HeLa cells, using 14-3-3 θ/τ (Phospho-Ser232) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using 14-3-3 θ/τ (Phospho-Ser232) antibody.