• Catalog Number:

  E1A0757A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human YAP around the phosphorylation site of serine 127 (A-H-SP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P46937

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  65 kDa Yes-associated protein, YAP1, YAP65

• Specificity Sensitivity:

  YAP (Phospho-Ser127) antibody detects endogenous levels of YAP only when phosphorylated at serine 127.

• References:

  Sudol M., J. Biol. Chem. 270:14733-14741(1995). Chen H.I., J. Biol. Chem. 272:17070-17077(1997). Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

• All Sites:

  Human: Ser127; Mouse: Ser112; Rat: Ser112

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using YAP (Phospho-Ser127) antibody. Western blot analysis of extracts from COS7 cells, treated with HU (2nM, 24hours), using YAP (Phospho-Ser127) antibody.