• Catalog Number:

  E1A0728A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ras-GRF1 around the phosphorylation site of serine 916 (R-M-SP-L-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Mouse, Rat

• SwissProtNo:

  Q13972

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  CDC25, GNRP, GRF1, Guanine nucleotide releasing protein, P140 Ras-GRF, RASGRF1, Ras-specific nucleotide exchange factor CDC25

• Specificity Sensitivity:

  Ras-GRF1 (Phospho-Ser916) antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

• References:

  Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

• All Sites:

  Mouse: Ser916; Rat: Ser898

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody.