Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PP1-α around the phosphorylation site of threonine 320 (P-I-TP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P62136
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
EC 3.1.3.16, PP-1A, PP1A, PPP1A, Serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit, Serine/threonine protein phosphatase PP1-alpha 1, catalytic subunit
Specificity Sensitivity:
PP1-α (Phospho-Thr320) antibody detects endogenous levels of PP1-α only when phosphorylated at threonine 320.
References:
Song Q., Gene 129:291-295(1993).
Ceulemans H., J. Biol. Chem. 277:47331-47337(2002).
Cohen P.T., J. Cell Sci. 115:241-256(2002).
All Sites:
Human: Thr320; Mouse: Thr320; Rat: Thr320
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PP1-α (Phospho-Thr320) antibody.