Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PLD1 around the phosphorylation site of serine 561 (K-F-SP-L-Y).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
PLD1 (Phospho-Ser561) antibody detects endogenous levels of PLD1 only when phosphorylated at serine 561.
References:
Nicholas Lehman, Blood, Nov 2006; 108: 3564 - 3572.
Yoe-Sik Bae, J. Immunol., Apr 2000; 164: 4089.
Maria Zeniou-Meyer, J. Biol. Chem., Jul 2007; 282: 21746 - 21757.
All Sites:
Human: Ser561; Mouse: Ser561; Rat: Ser561
P-peptide - +
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLD1 (Phospho-Ser561) antibody.
Immunofluorescence analysis of HepG2 cells, using PLD1 (Phospho-Ser561) antibody.