Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PKD1/PKC μ around the phosphorylation site of serine 910 (R-V-SP-I-L).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15139
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
EC 2.7.11.13, KPCD1, PKC-mu, PKCM, PKD, PRKCM, PRKD1, Protein kinase C, mu type, Protein kinase D, kinase PKD1, nPKC-mu
Specificity Sensitivity:
PKD1/PKC μ (Phospho-Ser910) antibody detects endogenous levels of PKD1/PKC μ only when phosphorylated at serine 910.
References:
Johannes F.-J., J. Biol. Chem. 269:6140-6148(1994).
Zemlickova E., Biochem. Biophys. Res. Commun. 307:459-465(2003).
Sjoeblom T., Science 314:268-274(2006).
All Sites:
Human: Ser910; Mouse: Ser916; Rat: Ser647
Western blot analysis of extracts from A431 cells, using PKD1/PKC μ (Phospho-Ser910) antibody.