• Catalog Number:

  E1A0720A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKD1/2/3/PKC μ around the phosphorylation site of serine 738and serine 742 (E-K-SP-F-R-R-SP-V-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15139

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.13, KPCD1, PKC-mu, PKCM, PKD, PRKCM, PRKD1, Protein kinase C, mu type, Protein kinase D, kinase PKD1, nPKC-mu

• Specificity Sensitivity:

  PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody detects endogenous levels of PKD1/2/3/PKC μ only when phosphorylated at serine 738and serine 742.

• References:

  Johannes F.-J., J. Biol. Chem. 269:6140-6148(1994). Zemlickova E., Biochem. Biophys. Res. Commun. 307:459-465(2003). Storz P., J. Biol. Chem. 278:17969-17976(2003).

• All Sites:

  Human: Ser738+Ser742; Mouse: Ser744+Ser748; Rat: Ser475+Ser479

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody. Western blot analysis of extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins), using PKD1/2/3/PKC μ (Phospho-Ser738+Ser742) antibody.