• Catalog Number:

  E1A0719A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC θ around the phosphorylation site of threonine 538 (T-N-TP-F-C).

• Purification:

  The antibody was affinity-purified frorabbitantiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q04759

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.13, KPCT, PKC-theta, PKCQ, PRKCQ, PRKCT, Protein kinase C, theta type, kinase PKC-theta, nPKC-theta

• Specificity Sensitivity:

  PKC θ (Phospho-Thr538) antibody detects endogenous levels of PKC θ only when phosphorylated at threonine 538.

• References:

  Baier G., J. Biol. Chem. 268:4997-5004(1993). Deloukas P., Nature 429:375-381(2004). Wissing J., Mol. Cell. Proteomics 6:537-547(2007).

• All Sites:

  Human: Thr538; Mouse: Thr538

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PKC θ (Phospho-Thr538) antibody. Western blot analysis of extracts from Jurkat cells, using PKC θ (Phospho-Thr538) antibody.