• Catalog Number:

  E1A0716A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKC α/β2 around the phosphorylation site of threonine 638 (V-L-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P17252

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha

• Specificity Sensitivity:

  PKC α (Phospho-Thr638) antibody detects endogenous levels of PKC α only when phosphorylated at threonine 638.

• References:

  Maria Nikodemova, J. Biol. Chem., May 2007; 282: 15208 - 15216. Pedro J. Cejas, J. Biol. Chem., Aug 2005; 280: 28412 - 28423. Dan Chen, J. Biol. Chem., Feb 2004; 279: 4829 - 4839. Nicole R. Murray, J. Cell Biol., May 1999; 145: 699.

• All Sites:

  Human: Thr638; Mouse: Thr638; Rat: Thr638

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC α (Phospho-Thr638) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with UV (15mins), using PKC α (Phospho-Thr638) antibody.