• Catalog Number:

  E1A0713A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Pin1 around the phosphorylation site of serine 16 (R-M-SP-R-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q13526

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 5.2.1.8; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; Rotamase Pin1

• Specificity Sensitivity:

  Pin1 (Phospho-Ser16) antibody detects endogenous levels of Pin1 only when phosphorylated at serine 16.

• References:

  Lu K.P., Nature 380:544-547(1996). Ebert L., Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Ser16; Mouse: Ser16

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using Pin1 (Phospho-Ser16) antibody. Western blot analysis of extracts from COS-7 cells, treated with insulin (0.01U/ml, 15mins), using Pin1 (Phospho-Ser16) antibody.