• Catalog Number:

  E1A0712A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Pim-1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P06803

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.11.1, PIM-1, Proto-oncogene serine/threonine-protein kinase pim-1

• Specificity Sensitivity:

  Pim-1 (Phospho-Tyr309) antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.

• References:

  Selten G., Cell 46:603-611(1986). Saris C.J., EMBO J. 10:655-664(1991). Maita H., Eur. J. Biochem. 267:5168-5178(2000).

• All Sites:

  Human: Tyr309; Mouse: Tyr302; Rat: Tyr218

Western blot analysis of extracts from HUVEC cells, treated with PMA (125ng/ml, 30mins), using Pim-1 (Phospho-Tyr309) antibody.