Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PEA-15 around the phosphorylation site of serine 104(I-P-SP-A-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15121
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
astrocytic phosphoprotein PEA-15; PE15; PEA15; PED; phosphoprotein enriched in diabetes
Specificity Sensitivity:
PEA-15 (Phospho-Ser104) antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.
References:
Estelles A., J. Biol. Chem. 271:14800-14806(1996).
Condorelli G., EMBO J. 17:3858-3866(1998).
Wolford J.K., Gene 241:143-148(2000).
All Sites:
Human: Ser104; Mouse: Ser104; Rat: Ser104
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody.
Western blot analysis of extracts from COS cells, treated with TNF (20ng/ml, 5mins), using PEA-15 (Phospho-Ser104) antibody.