• Catalog Number:

  E1A0710A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PBK/TOPK around the phosphorylation site of threonine 9 (F-K-TP-P-S).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q96KB5

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.12.2, MPAKK-like protein kinase cap site form 2, NORI-3, TOPK

• Specificity Sensitivity:

  PBK/TOPK (Phospho-Thr9) antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.

• References:

  Abe Y., J. Biol. Chem. 275:21525-21531(2000). Gaudet S., Proc. Natl. Acad. Sci. U.S.A. 97:5167-5172(2000). Zhao S., Int. J. Biochem. Cell Biol. 33:631-636(2001).

• All Sites:

  Human: Thr9

Western blot analysis of extracts from K562 cells, treated with UV (30mins), using PBK/TOPK (Phospho-Thr9) antibody.