• Catalog Number:

  E1A0707A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PAK2 around the phosphorylation site of serine 20 (M-S-SP-T-I).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13177

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  EC 2.7.11.1, Gamma-PAK, P21-activated kinase 2, PAK 2, PAK-2, PAK65, PAKI, S6/H4 kinase, kinase PAK2, p21-activated kinase 2, p21-activated protein kinase I

• Specificity Sensitivity:

  PAK2 (Phospho-Ser20) antibody detects endogenous levels of PAK2 only when phosphorylated at serine 20.

• References:

  Thomas Rudel, Science, Jun 1997; 276: 1571. Eun-Young Shin, J. Biol. Chem., Nov 2002; 277: 44417 - 44430. Jelena Mann, J. Immunol., Nov 2005; 175: 6560 - 6569. Zhongdong Huang, Mol. Cell. Biol., Feb 2004; 24: 1582 - 1594.

• All Sites:

  Human: Ser20; Mouse: Ser20; Rat: Ser20

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PAK2 (Phospho-Ser20) antibody. Western blot analysis of extracts from 293 cells, treated with Sorbitol (0.4M, 30mins), using PAK2 (Phospho-Ser20) antibody.