• Catalog Number:

  E1A0703A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p63 around the phosphorylation site of serine 455 (I-Q-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  Q9H3D4

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EEC3, KET, LMS, SHFM4, TA p63 alpha, TP63, p51, p73H, p73L, tumor protein p63

• Specificity Sensitivity:

  p63 (Phospho-Ser455) antibody detects endogenous levels of p63 only when phosphorylated at serine 455.

• References:

  Senoo M., Biochem. Biophys. Res. Commun. 248:603-607(1998). Augustin M., Mamm. Genome 9:899-902(1998). Yang A., Mol. Cell 2:305-316(1998).

• All Sites:

  Human: Ser455

Western blot analysis of extracts from HeLa cells, treated with TNF (2500U/ml, 30mins), using p63 (Phospho-Ser455) antibody.