Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human p40 phox around the phosphorylation site of threonine 154 (P-R-TP-R-K).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
p40 phox (Phospho-Thr154) antibody detects endogenous levels of p40 phox only when phosphorylated at threonine 154.
References:
S Zhan, Blood, Oct 1996; 88: 2714 - 2721.
Ahmad Utomo, J. Immunol., Nov 2006; 177: 6388 - 6397.
Sen-Lin Li, Blood, May 2002; 99: 4578 - 4587.
Rong He, J. Immunol., Dec 2004; 173: 7462 - 7470.
All Sites:
Human: Thr154; Mouse: Thr154
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using p40 phox (Phospho-Thr154) antibody.